Fig. 2. Contents of amino acid produced from glutamine metabolism in C₂C₁₂ myotubes cultivated in various glutamine concentrations (0, 2, 8 or 16 mM) during 48 hours. The Fig. refers to the sum of amino acids derived from glutamine metabolism (Gln, Glu, Asp, Asn, Ala, Pro, Orn, and Arg). The experimental groups are: (0 mM) no glutamine addition in the medium; 2 mM (reference condition), 8 mM or 16 mM L-glutamine concentration in the medium. The values are as the mean ± standard error of three independent experiments. Results were analyzed using one-way ANOVA and Tukey post-test. (^#p<0.001) compared with 2 mM glutamine.